Table 1. Bacterial strains and plasmids used in this study.
| Strains or plasmids | Description | Source |
| Bacteria | ||
| K. pneumoniae strains | ||
| K. pneumoniae isolates (74) | Clinical isolates collected from National Taiwan University Hospital during 1997–2003 | [4], [23] |
| NTUH-K2044 | Clinically isolated strain causing PLA, the parental strain for generation of isogenic mutants | [30] |
| ΔsugE | NTUH-K2044 isogenic mutant with deletion of sugE gene | This study |
| ΔsugE/sugE+ | NTUH-K2044 ΔsugE with sugE cassette between pgpA and yajO | This study |
| ΔtreC | NTUH-K2044 isogenic mutant with deletion of treC gene | This study |
| ΔtreC/treC+ | NTUH-K2044 ΔtreC with treC cassette between pgpA and yajO | This study |
| Δwza | NTUH-K2044 isogenic mutant with deletion of wza gene | This study |
| ΔtreB | NTUH-K2044 isogenic mutant with deletion of treB gene | This study |
| ΔlacZp | NTUH-K2044 isogenic mutant with deletion of lacZ promoter | [28] |
| E. coli strains | ||
| DH10B | F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80 δλαχZ ΔM15 ΔlacX74 endA1 recA1 deoR (ara leu)7697 araΔ139 galU galK nupG rpsL λ− | Invitrogen |
| Plasmids | ||
| pGEM-T easy | TA cloning vector | Promega |
| pKO3-Km | pKO3-derived plasmid, with a kanamycin-resistant cassette inserted in AccI site | [26] |
| pKO3-Km-pgpA-yajO | pKO3-Km derived plasmid, with a region containing part of the thiL, the yajO, and part of the phosphatidyl- glycerophosphatase A genes inserted into NotI site | [28] |
| pPCR2.1-TOPOII-GFP-CAT | GFP-expressing plasmid | [30] |
NOTE. K. pneumoniae, Klebsiella pneumoniae; E. coli, Escherichia coli; PLA, pyogenic liver abscess; Km, kanamycin; GFP, green fluorescence protein.