Figure 3. PARP1 depletion does not impair the initial recruitment of condensin I but affects its retention at the damage sites.

(A) Cells were treated with siRNA specific for PARP1 or control siRNA, and stained with antibodies specific for PAR, XRCC1, PARP1, or hCAP-G at five min after damage induction. (B) Cells were treated with PARP1 or control siRNA as in (A) and the accumulation of GFP-hCAP-G was plotted over time as in Fig. 1D. (C) Cells treated with NU1025 were also treated with PARP1 or control siRNA and the accumulation of the endogenous hCAP-G and PARP1 were examined at one hour after damage induction.