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. 2011 Feb 17;21(9):2177–2186. doi: 10.1093/cercor/bhr006

Figure 1.

Figure 1.

Neural differentiation from Fezf2 overexpression mESCs. (A) The treatment of Dox induced high Fezf2 mRNA expression in the Fezf2 overexpression ESC clones. (B) qPCR data indicating that when Dox was added, there was more than a 20-fold increase in the Fezf2 expression. (C) The induction of Fezf2 protein was observed in the Fezf2-inducible ESC line after Dox treatment using western blotting. (D) A schematic procedure for neural differentiation from mESCs. (E) qPCR indicating the gene expression profiles at different time points during neural differentiation from mESCs. (F) At day 8 after differentiation, immunostaining showed that the addition of Dox yielded no significant changes in the expression of NESTIN and SOX1. Bar = 50 μm. The overexpression of Fezf2 also did not affect the expression of Nestin and Sox1 mRNA as shown by regular PCR (day 6, G) and qPCR (day 6, H). Mean ± SD, n = 3. *P < 0.05 versus Control (No Dox) by 2-sided t-test.