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. 2011 Feb 17;21(9):2177–2186. doi: 10.1093/cercor/bhr006

Figure 4.

Figure 4.

The establishment and differentiation of Fezf2 mESC knockdown mESC lines. (A) Fezf2 and luciferase RNAi sequences were inserted into the PLKO.1 vector and lentivirus was produced. (B) mESCs were transduced with the lentivirus. The RNAi-expressing cells, as indicated by the expression of GFP, were then differentiated into EBs and NE cells. (C) The expression of Fezf2 was significantly decreased in the Fezf2 knockdown mESC lines. The Fezf2 expression remained very low when the cells were differentiated for 10 days. (D) The Fezf2 knockdown cell line showed a decrease in Foxg1 and Six3 mRNA expression and an increase in En1 expression relative to the luciferase RNAi cell line at day 10. (E) RT-PCR showed that the expression of Nestin and Sox1 mRNA was similar between control and Dox-treated cells at day 6 after differentiation. Mean ± SD, n = 4. *P < 0.05 versus Control by 2-sided t-test. Bar = 50 μm.