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. 2011 Feb 17;21(9):2177–2186. doi: 10.1093/cercor/bhr006

Figure 5.

Figure 5.

Changes in neural differentiation after Fezf2 overexpression at an early time point. (A,B) The overexpression of Fezf2 on days 4–6 lead to an increase in the expression of both dorsal (Pax6, Emx1) and ventral (Nkx2.1 and Dlx2) forebrain transcriptional factors at both the mRNA (A) and the protein (B) levels at day 10 after differentiation. (C) Statistical analysis showed that there was a significant increase in the population of NKX2.1+ and PAX6+ cells after Dox treatment. (D) Upon further differentiation, Dox treatment resulted in an increased expression of Islet1, Tbr1, and BCL11B (Ctip2) proteins. Glutamatergic neurons which stained positive for Tbr1 were also positive for the neuronal markers, βIII-tubulin (D) and MAP2 (E). Some cells were positive for GABA (E). Blue indicated Hoechst-stained nuclei. Mean ± SD, n = 4. *P < 0.05 versus Control by 2-sided t-test. Bar = 50 μm.