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. 2011 Feb 17;21(9):2177–2186. doi: 10.1093/cercor/bhr006

Figure 6.

Figure 6.

The time-dependent effect of Fezf2. (A) A schematic demonstrating the time points during which cells were exposed to Dox. (B) The expression levels of different D–V markers were evaluated at day 15 via qPCR. When the Fezf2-inducible overexpression cell lines were exposed to Dox during both the early and the late stages of differentiation, there was an increase in the expression of both dorsal telencephalic progenitor and deep-layer cortical projection neuron markers. Moreover, the expression of ventral forebrain and upper cortical layer markers were decreased when Fezf2 was overexpressed at both early and late stages. (C,D) Immunostaining of the Fezf2-inducible overexpression cells was evaluated and it showed that there was a similar trend in the expression of dorsal forebrain progenitor (PAX6+ cells), ventral forebrain progenitors (NKX2.1+ cells), and layer V projection neurons (OTX1+ cells). Mean ± SD, n = 3. *P < 0.05 versus No Dox Control by 2-sided t-test. Blue indicated Hoechst-stained nuclei. Bar = 50 μm.