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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Protein Expr Purif. 2011 Jun 13;79(2):277–284. doi: 10.1016/j.pep.2011.05.022

Fig. 1.

Fig. 1

Expression and purification of Sindbis virus nsP1 from E. coli. SDS-PAGE gel stained with Coomassie Blue. Lanes: 1, molecular mass standards (kDa); 2, Uninduced insoluble fraction of E. coli Rosetta (DE3); 3, Uninduced soluble fraction of lysed Rosetta(DE3); 4, Induced insoluble fraction of lysed Rosetta(DE3); 5, Induced soluble fraction of Rosetta(DE3) containing nsP1; 6, Purified full length (~ 60 kDa) SINV nsP1.