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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Protein Expr Purif. 2011 Jun 13;79(2):277–284. doi: 10.1016/j.pep.2011.05.022

Fig. 4.

Fig. 4

Autoradiograph of SDS-PAGE gel showing the formation of α-32P labeled m7GMP-nsP1 covalent complex. The reaction mixture included the standard components as described in Materials and Methods with 5 µCi of [α-32P] GTP, and ~25 pmol/100 pmol of nsP1. Incubation was at 30 °C for 30 min. A. Arrows indicate position of prestained molecular weight markers; Lane 1, 25 pmol nsP1; 2, 100 pmol of nsP1; 3, no protein; 4, no AdoMet; 5, Positive control vaccinia virus capping enzyme.