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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Protein Expr Purif. 2011 Jun 13;79(2):277–284. doi: 10.1016/j.pep.2011.05.022

Fig. 6.

Fig. 6

Effect of NaCl on enzymatic activities of SINV nsP1. A. Effect of NaCl on MTase activity. Transfer of [3H]-methyl from AdoMet to GIDP in the presence of increasing concentrations of NaCl. B. Autoradiograph of SDS-PAGE gel showing the formation of α-32P labeled m7GMP-nsP1 covalent complex in the presence of varying concentrations of NaCl. Lanes: 1, 0 mM NaCl; 2, 25 mM NaCl; 3, 50 mM NaCl; 4, 100 mM NaCl; 5, 200 mM NaCl; 6, 300 mM NaCl; 7, 500 mM NaCl.