Skip to main content
. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Mech Ageing Dev. 2011 May 27;132(6-7):274–286. doi: 10.1016/j.mad.2011.05.003

Figure 6. Cytokine dysregulation in aged splenic macrophages stimulated with TLR2 ligands can be partially rescued by inhibiting PI3K.

Figure 6

The graphs show cytokine secretion by purified aged and young adult splenic macrophages pretreated with LY294002 for 60 minutes and then stimulated with Pam2CSK4 (Panels A and B), LTA (1µg/ml) (Panels A and C) for 24 hours. Supernatants were collected and assayed for IL-10 (Panel A) or IL-12(p40) (Panels B and C) by sandwich ELISA. Data are presented as mean +/− SE of duplicate cultures and duplicate ELISA’s. The symbols * and # indicate statistical significance of responses in the aged splenic macrophages treated with Pam2CSK4 or LTA with or without the PI3K inhibitor.