Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Traffic. 2011 Jul 3;12(9):1242–1256. doi: 10.1111/j.1600-0854.2011.01227.x

Figure 1. Live cell imaging of KATP channel endocytosis.

Figure 1

(A) Plasma membrane bound KATP channels are visible immediately after adding TRITC-BTX protein ligand dye in a COSm6 cell transiently transfected with BTX tag-SUR1 and WT Kir6.2 (picture acquisition start, scale bar 5 μm). Channels budding from the plasma membrane and migrating towards the cytosol are visible as bright fluorescent puncta (white arrowheads in 10-30min movie captures, white arrow and inset at 30min). At the start of acquisition only surface-bound channels are depicted as two peaks in the line scan graph (A, bottom part, x-axis at approximately 5 and 15 μm). Internalized channels are illustrated as peaks around 10 μm on the x-axis of the graph corresponding to the inside of the cell (red arrows). (B) Similar observations were made in INS-1 cells infected with adenoviral BTX tag-SUR1 and WT Kir6.2 (B, white arrowheads, arrow and inset, red arrows in graph). KATP channel endocytosis was visually followed over 30min and internalization was illustrated in the graphs below the movie snap shots (bottom part, corresponding movies in supplemental data).