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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Traffic. 2011 Jul 3;12(9):1242–1256. doi: 10.1111/j.1600-0854.2011.01227.x

Figure 7. Endocytosis of SUR1RKR→AAA is clathrin-mediated.

Figure 7

Cells expressing WT channels or SUR1RKR→AAA were treated with different inhibitors of CME (see Materials and Methods). Internalization of WT and SUR1RKR→AAA was inhibited substantially when the cells were treated with 10μg/ml chlorpromazine (middle). WT channel internalization was blocked by 91.9% (n=16) and SUR1RKR→AAA by 95.9% (n=14) compared to their respective untreated controls (top; n>10). Transferrin uptake was almost completely blocked under these conditions (green). Exposure to potassium free solution before and during the assay (bottom) also blocked internalization of both WT (by 98.1%; n=5) and SUR1RKR→AAA (by 98.1%; n=8) compared to their respective untreated controls (n>10). Scale bars: 10μm.