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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Inorg Biochem. 2011 Jun 24;105(9):1226–1237. doi: 10.1016/j.jinorgbio.2011.06.003

Figure 4. Fluorescence of T34C/T110W CaM and its complexes with NOS target peptides.

Figure 4

Each spectrum is the average of two scans obtained at room temperature and the fluorescence intensities are in arbitrary units. Buffer: 50 mM HEPES, pH 7.8 with 100 mM NaCl and 50 µM EDTA. 8.7µM Apo T34C/T110W CaM (open circles) was first mixed with (A) 39.3 µM nNOS726–749 (B) 35.3 µM eNOS492–511 or (C) 31 µM iNOS507–531 (solid triangles); then mixed with 450 µM CaCl2 (plus) and finally mixed with 4 mM EDTA (stars).