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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Inorg Biochem. 2011 Jun 24;105(9):1226–1237. doi: 10.1016/j.jinorgbio.2011.06.003

Figure 5. Fluorescence of Alexa-CaM and its complexes with NOS target peptides.

Figure 5

The change of FW110 between 300 – 390 nm at room temperature is shown. Each spectrum is the average of two scans and the fluorescence intensities are in arbitrary units. Buffer: 50 mM HEPES, pH 7.8 with 100 mM NaCl and 100 µM EDTA. A – C: apo 53 nM Alexa-CaM (open circles) was first mixed with (A) 3.4 µM nNOS726–749 (B) 3.4 µM eNOS492–511 or (C) 3.1 µM iNOS507–531 (solid triangles); then mixed with 300 µM CaCl2 (plus) and finally mixed with 2 mM EDTA (stars). D: apo 53 nM Alexa-CaM (open circles) was first mixed with 300 µM CaCl2 (solid diamonds); then mixed with 3.1 µM iNOS507–531 (cross) and finally with 2 mM EDTA (stars).