Table 1.
kon and koff rate constants of NOS target peptides to holo Alexa-CaM.
| kon (M−1s−1)a | koff (s−1)b | Kd, apparent (nM)c | Kd (nM)d | |
|---|---|---|---|---|
| nNOS726–749 | 6.6 (± 0.6) × 108 | 3.7 ± 0.1 | 5.6 | 1 – 5.0 [18, 19, 21] |
| eNOS492–511 | 2.9 (± 0.1) × 108 | 4.5 ± 0.3 | 1.6 | 2.9 – 4.0 [16, 23] |
| iNOS507–531 | 6.1 (± 0.1) × 108 | 0.063 ± 0.007 | 0.1 | < 0.1 – 3.3 [12, 16, 21–23] |
a
kon constants were measured with fluorescence stopped-flow at 4.5 °C.
b
koff constants were measured with fluorescence stopped-flow at 4.5 °C.
c
Kd, apparent was calculated as koff /kon.
d
From literatures.