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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Genes Chromosomes Cancer. 2011 Jul 13;50(10):830–836. doi: 10.1002/gcc.20904

Figure 1. Expression of 14-3-3σ is increased in human lung tumors.

Figure 1

(A) Total RNA was extracted from frozen specimens of normal and tumor tissues and the quantity of 14-3-3σ was determined using quantitative RT-PCR. Relative quantification (−ΔCT) values were determined by comparison with GAPDH. The box plots depict relative quantities of 14-3-3σ in normal and tumor tissues. p-values are for comparison of the levels of 14-3-3σ mRNA in tumor specimens with normal tissue and is calculated using ΔCt values. (B) Protein extracts prepared from the same tumor samples as in A were fractionated on SDS-PAGE protein gels, blotted and probed for 14-3-3σ protein using mouse monoclonal 14-3-3σ antibody (sc-100638, Santa Cruz, CA). Typical results for four normal and four tumor specimens are shown. β-actin was used as a loading control.