Table 4.

Quantitative trait loci (QTLs) associated with necrosis (N) and pycnidia development (P) in the Apache/Balance DH population after inoculation with five Mycosphaerella graminicola isolates in the seedling stage

Isolate	Closest marker	Chromosome position	Phenotypic data seta	Resistance source	N			P
					PDb (cM)	LOD	Exp. (%)	PD (cM)	LOD	Exp. (%)
IPO323	wPt-0836	3AS	R1	Balance	0	12.2	46.1	1	7.3	27.7
			R2		1	25.5	73.7	1	11.1	39.7
			R3		1	25.6	73.1	1	10.8	38.7
	Xgpw5176-Xgpw3087 c	6DS	R1	Balance				3.2–5	3.6	12.7
			R2					0.3–8	3.1	8.9
			R3					4.3–4	3.5	11
IPO98022	Xgpw5176-Xgpw3087 c	6DS	R1	Balance	6.3–2	6.4	30.4	5.3–3	16.3	47
			R2		8.3–0	5.4	21.6	5.3–3	13.1	47.4
			R3		0.3–8	4.4	18.8	5.3–3	12.3	48
	Xgwm111	7DS	R1	Apache				1.1	6.2	11.8
			R2					0	5.2	11.2
			R3					0	2.2d	5.9
IPO89011	Xgpw5176-Xgpw3087 c	6DS	Ave.	Balance				5.3–3	23.16	67.5
	wPt-1859	7DL	Ave.	Apache				0	4.5	8
IPO98046	Xgwm111	7DS	R1	Apache				0	9.5	27.5
			R2					−e	–	–
			R3					0	6.2	20.8
	Xgpw313	7DL	R1	Apache				–	–	–
			R2					5	6.8	20.5
			R3					–	–	–
			Ave.
	Xgpw5176-Xgpw3087 c	6DS	R1	Balance				8.3–0	7	19
			R2					8.3–0	7.9	24.2
			R3					6.3–2	7.4	27.1
IPO87016	wPt-2019	1BS	R1	Apache				2	19.1	63.3
			R2					1	21.1	68.3
			R3					0	17.8	59.3
			Ave.		1	21.11	67.3

^aR1, R2 and R3 represent first, second and third replicate data. QTL analysis was performed on averaged data (Ave) when Bartlett’s χ ² test indicated nonsignificant phenotypic variation over replicates, otherwise replicates were processed individually

^bPD, QTL peak distance in cM

^cFlanking markers

^dNot significant, but consistent QTL position

^eNo QTL detected