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. Author manuscript; available in PMC: 2011 Aug 14.
Published in final edited form as: Synapse. 2010 Apr;64(4):267–273. doi: 10.1002/syn.20727

Fig. 4.

Fig. 4

DADLE or naltrexone tends to enhance differentiation as indicated by the expression of neural marker β-III-tubulin. AF5 cells were cultured to subconfluency in the DMED/F12 medium (FBS) or in the Neurobasal/B27 differentiation medium (NB) post-confluency for 4 days. DADLE, naltrexone, or DADLE plus naltrexone were present in the differentiation medium during the observation period of 4 days. (A) Western blottings indicate the tendency of an increased expression of neuron specific β-III-tubulin (TuJ-1) in DADLE (0.1 nM)- or naltrexone (0.1 nM)-treated cells. (B) Statistic analysis of β-III-tubulin expression. β-III-tubulin protein expression was normalized to GAPDH. n = 3. *P < 0.05 and **P < 0.01 compared to differentiated samples (NB). P = 0.09 between NB and DADLE.