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. Author manuscript; available in PMC: 2011 Aug 14.
Published in final edited form as: Antiviral Res. 2008 Jun 20;80(2):114–123. doi: 10.1016/j.antiviral.2008.05.010

Figure 3.

Figure 3

Assay to eliminate trivial inhibitors. Hit compounds from the primary screen using vaccinia lysates were futher tested in a KSHV rapid plate assay for their abilities to inhibit DNA synthesis directed by Pol8/PF8. Compounds that inhibited Pol8/PF8 DNA synthesis (solid bars) were categorized as potentially trivial inhibitors. Compounds that failed to block Pol8/PF8 DNA synthesis (shaded bars) were considered to be bona fide inhibitors of vaccinia DNA synthesis. Indicated are wells with the positive (+) control inhibitor EDTA (E) in duplicate and the negative (-) control inhibitor DMSO alone (D) and no compound added (N).