Table 2.

Protein denaturation summary for interactions of vaccinia virus D4 with small molecule compounds^a

Hit	Tm (°C)	ΔTm (°C)	Hit	Tm (°C)	ΔTm (°C)
DMSO mock	38.4 ± 1.6	0.0	14	48.4 ± 6.0	10.0
1	34.7 ± 1.5	−3.7	15	44.3 ± 3.7	4.9
2	36.4 ± 0.4	−2.0	16	35.2 ± 2.0	−5.0
3	35.1 ± 0.7	−3.3	17	35.1 ± 1.8	−1.7
4	35.2 ± 1.0	−3.2	18	34.3 ± 0.7	−4.1
5	36.4 ± 1.3	−2.0	19	38.3 ± 1.3	−0.1
6	36.9 ± 1.0	−1.5	20	34.9 ± 1.3	−3.5
7	36.3 ± 0.9	−2.1	21	34.8 ± 1.5	−3.6
8	32.2 ± 0.3	−6.6	22	34.7 ± 0.4	−3.7
9	51.4 ± 9.1	13.1	23	33.2 ± 0.3	−4.9
10	53.3 ± 1.5	14.9	24	35.4 ± 0.4	−3.2
11	36.3 ± 0.5	−1.5	25	35.9 ± 0.8	−2.5
12	35.0 ± 0.4	−3.4	26	33.6 ± 2.0	−4.8
13	36.5 ± 1.2	−1.9	27	43.0 ± 3.0	4.6

^aExperiments were performed using 4 μM D4 in the presence or absence of 50 μM compounds in 25 mM Tris, pH 7.2, 0.15 M NaCl, 5 mM ZnCl₂, and 1 % DMSO. T_m values are shown ± SD from at least three independent experiments (n=3-5), with thermal shift (ΔT_m) values obtained by the subtraction of the untreated DMSO mock.