Figure 4.

Parasite maturity during the time-course experiment. Sorbitol lysis was carried out at 12 hours after the first ring invasion was seen (time point one), and samples were then taken 8-hourly throughout the asexual blood stage cycle. The maximum parasite maturity in terms of hours post invasion at each time point is shown in the second column. Samples for RNA extraction were taken from the culture at each time point, mixed with TRIzol reagent and frozen, and a Giemsa-stained thin blood smear was performed to record the developmental stage of rosetting (R29R+) and non-rosetting (R29R-) parasites.