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. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: Mol Cell. 2011 Jul 22;43(2):229–241. doi: 10.1016/j.molcel.2011.05.029

Figure 2.

Figure 2

The yeast and mouse p23 proteins are negative modulators of protein transport. (A) Colocalization of mouse p23 (red) with the Golgi maker protein Giantin (green) was determined by IIF. The cells were counterstained with DAPI to detect the nuclei. (B) The impact of Sba1 levels on yeast Invertase secretion was determined. The influence of Sba1 loss (sba1Δ), Sba1 overexpression or Sba1Δ84 (chaperone mutant) expression was checked as well as the secretion defective mutant sec18-1. The levels of intracellular Myc-Invertase were detected by immunoblot analysis. (C) The efficiency of Preprolactin processing to Prolactin during transport in parental and p23 null MEF cells was determined. (D) The effect of Sba1 overexpression on Fus-Mid-GFP transport was examined by fluorescence microscopy in WT and secretion compromised yeast.