Table 2.
Diarrhea and fecal virus shedding in calves after oral inoculation with BRV IND (P[5]G6).
| Treatment Group |
n | Inoculum infectious titer (FFU/ml) | Diarrheab,e | Virus sheddinga | Days with fever asoc to BRV (>39ºC) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % Affected Calvesd | Mean Onset (PID) | Mean Duration (days) | Mean Cumulative Scorec | % Affected Calvesd | Mean Onset (PID) | Mean Duration (days) | Mean Peak shed virus (CCIF) | Mean RV titer (CCIF) | ||||
| Gp 1: CC + IND BRV IgY supplemented milk | 5 | 10 4.76 | 20% A | 4.0 | 0.4 B | 2.00 B | 80% | 2.00 | 5.2 | 5.27 | 4.83 AB | 0.2 C |
| Gp 2: CC + CONTROL IgY supplemented milk | 6 | 10 4.79 | 100% B | 3.3 | 5.2 AB | 12.89 AB | 100% | 1.75 | 6.7 | 6.47 | 6.27 A | 1.3 BC |
| Gp 3: CC + Ab free milk | 9 | 10 4.55 | 100% B | 2.2 | 7.4 A | 20.22 A | 100% | 2.11 | 8.0 | 5.36 | 3.98 B | 7.4 A |
| Gp 4: CD + Ab free milk | 8 | 10 4.12 | 100% B | 1.1 | 9.4 A | 26.88 A | 100% | 1.50 | 5.5 | 5.71 | 4.45 AB | 4.4 AB |
Determined by ELISA and CCIF.
Diarrhea duration was defined as the number of days with fecal score ≥2. Stool consistency was scored daily (0=normal; 1=pasty; 2=semi-liquid; 3=liquid).
Mean cumulative scores from 0 to 21 PID calculated as a measure of severity of diarrhea (sum daily fecal score)/n.
Proportions in the same column with different superscript upper case letters differs significantly (Fisher’s exact test, p<0.005).
Means in the same column with different superscript upper case letters differ significantly (Kruskall-Wallis rank sum test; p<0.05).
CC= control colostrum / CD = colostrum deprived. Calves were fed with 2 liters of milk, twice a day, for 14 days, with or without the addition of the corresponding volume of IND BRV IgY pool or control IgY pool, according to the group.