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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Thromb Haemost. 2009 Oct;102(4):623–633. doi: 10.1160/TH09-04-0238

Figure 6. The time courses of human prethrombin-1, prethrombin-2, fragment 2 and the B-chain during prethrombin-1 activation by prothrombinase in the presence of A) human or B) bovine factor Va.

Figure 6

Pre-1 (1.4μM) was incubated with either human or bovine FVa (10nM), CaCl2 (2.0mM), PCPS vesicles (20μM) and DAPA (3.0μM) in 0.02M HEPES, 0.15M NaCl, pH 7.4 and 0.01% (v/v) Tween-80, and its activation was initiated by the addition of FXa (2.5nM). Samples were withdrawn at various times, and were analyzed as in Figure 5.