I.	Xylene wash	3-5 minutes
II.	Xylene	3-5 minutes
III.	100% alcohol	3-5 minutes
IV.	100% alcohol	3-5 minutes
V.	95% alcohol	3-5 minutes
VI.	80% alcohol	3-5 minutes
VII.	Water wash (several changes)	2 minutes
VIII.	Gill's Hematoxylin #3	2-5 minutes
IX.	Water wash (several changes)	until water runs clear
X.	Scott's water (to blue nuclei)	3 minutes or more
XI.	Water wash (several changes)	1-2 minutes
XII.	Eosin Y	1 minute
XIII.	Water wash (several changes)	30 seconds
XIV.	80% alcohol	1 minute
XV.	95% alcohol	2 minutes
XVI.	95% alcohol	2 minutes
XVII.	100% alcohol	3 minutes
XVIII.	100% alcohol (clean)	3 minutes
XIX.	Xylene	2 minutes or more
XX.	Xylene	2 minutes or more