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. 2011 Jun 16;118(6):1653–1662. doi: 10.1182/blood-2010-11-316943

Figure 5.

Figure 5

Concentration dependence of trypsin digestion of Glu-Pg. Glu-Pg was reduced and alkylated and either untreated (0) or digested with the indicated weight ratio of trypsin to Glu-Pg for 1 hour and coated onto wells of microtiter plates as described in “Methods.” Reactivity with mAb 51 (□; A), mAb 109 (□; B) and mAb 116 (□; C) was determined by ELISA as described in “Epitope determination: ELISA analysis of trypsin digests of Pg” and compared with the isotype control, MOPC21 (■ in all panels). *The trypsin:Glu-Pg ratio selected for study of each mAb.