Figure 3. Effect of TCDD administration in vivo on Foxp3 and IL-17 expression in lymphoid organs during colitis.
MLN, and LP cells were isolated on day 14 of colitis from the 4 groups of mice as described in Fig. 1. RT-PCR was used to detect mRNA levels for Foxp3 and IL-17 (panel A) and data from 3 independent experiments in each group is shown in panel B. Vertical bars represent mean+/− SEM of three independent experiments. Comparisons were made between VEH vs DSS+VEH groups as well as between DSS+VEH vs DSS+TCDD groups. Asterisks (*) represent statistically significant (p<0.05) difference between VEH and DSS+VEH groups and between DSS+VEH and DSS+TCDD groups.