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ERαAF2 is necessary for the E2 uterotrophic action. (A) Representative Western blot of ERα and β-actin protein levels (Upper). Proteins from uteri of 10-wk-old ERαAF2+/+ and ERαAF20 nonovariectomized mice were loaded as described in Methods. Protein levels (n = 4 for each genotype) were quantified using ImageJ software (Lower). (B) Uterine weight from ERαAF2+/+ and ERαAF20 ovariectomized mice treated or not treated with E2 (80 μg/kg per d).
