Fig. 4.
ERαAF2 is dispensable for the effect of E2 on endothelial healing. (A) Representative en face confocal immunohistochemical analysis of the intima tunica of the carotid artery from an E2-treated mouse 72 h after surgery with designation of the regenerative endothelial area. Nuclei, stained with propidium iodide, appear in dark blue. (B) The effect of E2 was studied in the ERαAF2+/+ and ERαAF20 ovariectomized mice. Quantification (mean ± SEM) of the regenerative endothelial area was from an average of four mice per group. Analysis by a two-way ANOVA: effect of exogenous E2, P = 0.01; effect of genotype, not significant (P = 0.78); interaction, not significant (P = 0.45).