Fig. 5.

ERαAF2 is necessary for the effect of exogenous E2 on fatty streak deposit prevention in 18-wk-old mice. Four-week-old ovariectomized ERαAF2^+/+LDL-r ^−/− and ERαAF2⁰LDL-r ^−/− mice were given either placebo or E2 (80 μg/kg per d during 12 wk) and switched to an atherogenic diet from the age of 6–18 wk. (A) Representative micrographs of oil red-O lipid-stained cryosections of the aortic sinus. (B) Quantification of lesion area at the aortic sinus. Analysis was performed with a two-way ANOVA; because an interaction was observed between the two factors, effect of E2 treatment was studied in each genotype using a Bonferroni posttest (***P < 0.001).