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Phosphorylation of Stat3 in human cell lines. MCF-7, HCC-1954, T-47D, and SK-BR-3 cells were treated with 1 μM GDC-0941, 5 nM rapamycin, or 20 μM LFM-A13 for 24 h, as indicated. Cells were then lysed and analyzed by Western blotting. MCF-7 and SK-BR-3 show significant reduction in Stat3 phosphorylation in response to both PI3K and to Tec kinase inhibition, similar to the sensitivity of 10T1/2-H1047R cells.
