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. 2011 Jul 25;108(32):13130–13134. doi: 10.1073/pnas.1110081108

Fig. 4.

Fig. 4.

WHSC1 controls histone H4K20 methylation and 53BP1 localization in response to DNA replication stress. (A) Impaired localization of 53BP1 to sites of replication stress in the absence of WHSC1. HCT116 cells expressing FF, WHSC1 shRNAs, or WHSC1 shRNA together with an shRNA-resistant WT or SET mutant WHSC1 cDNA were treated with 500 μM HU for 24 h and processed for immunostaining to visualize p-H2AX (red) and 53BP1(green). Nuclei were stained with DAPI. (B) WHSC1 regulates the methylation status of H4K20 upon DNA replication stress. HCT116 cells expressing FF or WHSC1 shRNAs were treated with 500 μM HU. Cells were released into HU-free media and collected, lysed at the indicated times, and immunoblotted to determine H4K20 methylation. GADPH was used as a loading control.