Fig. 3.

Effects of the extension of the terminator stem on the properties of SgrS. (A) Nucleotide sequences around the rho-independent terminators of SgrS-LS4U and SgrS-LS8U. The bold letters represent the bases corresponding to the extended stem. The inverted repeat sequences are underlined. (B) Expression and function of SgrS-LS4U and SgrS-LS8U. T1568 and TM589 cells harboring indicated plasmids were grown in LB medium. At A₆₀₀ = 0.6, total RNAs were prepared, and 0.25 or 7 μg of each RNA sample was subjected to Northern blot analysis using the SgrS probe 1 or the ptsG probe. Total proteins were also prepared and samples equivalent to 0.025 A₆₀₀ units were subjected to Western blot analysis using anti-IIB antibodies.