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. 2011 Jun 13;108(32):E431–E439. doi: 10.1073/pnas.1105876108

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Fig. 6. — EMSAs with Nla6-DBD and Nla28-DBD as well as the promoter region of the nla28 operon. (A) nla28 promoter region. The location of the σ⁵⁴ promoter is indicated by the arrow. The lengths of the P1 and P2 fragments are shown in parentheses. The sequences and locations of the putative EE1 and EE2 enhancer element half sites are shown above their corresponding promoter fragment. EMSAs were performed using Nla28-DBD or Nla6-DBD and end-labeled P2 promoter fragment (B) or Nla6-DBD or Nla28-DBD and end-labeled P1 promoter fragment (C). A 100-fold excess of the following unlabeled promoter fragments (cold DNA) was added for competition assays: P2 (lane 6), dev (lane 7), P2⁽⁶⁰⁾ (lane 8), and P2^(60)* (lane 9) (all in B) and P1 (lane 6), dev (lane 7), P1⁽⁶⁰⁾ (lane 8), and P1^(60)* (lane 9) (all in C).