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. 2011 Jun 13;108(32):E431–E439. doi: 10.1073/pnas.1105876108

Fig. 7.

Fig. 7.

EMSAs using Nla6-DBD, Nla28-DBD, and ActB-DBD as well as the promoter region of the actB operon. (A) actB promoter region. The location of the σ54 promoter is indicated by the arrow. The lengths of the P1 and P2 fragments are shown in parentheses. The sequences and locations of the putative EE1, EE2, and EE3 enhancer element half sites are shown above their corresponding promoter fragment. EMSAs were performed using Nla28-DBD or Nla6-DBD proteins and end-labeled P1 promoter fragment (B); Nla28-DBD, Nla6-DBD, or both Nla28-DBD and Nla6-DBD and end-labeled P1 promoter fragment (C); or ActB-DBD and end-labeled P2 promoter fragment (D). A 100-fold excess of the following unlabeled promoter fragments (cold DNA) was added for the competition assays shown in B: P1 (lanes 4 and 7) or dev (lanes 3 and 8). In the competition assays shown in D, a 50-fold excess of unlabeled P2 (lane 3), a 100-fold excess of unlabeled P2 (lane 4), a 200-fold excess of unlabeled P2 (lane 5), or a 200-fold excess of unlabeled dev (lane 6) was added.