Table 1.

Developmental phenotypes of EBP mutants^*

Strain	Aggregation†	Spore no.‡	Viable spore no.§
WT	+	100.0 ± 8.5	100.0 ± 19
actB	±	1.2 ± 0.8	<0.001
nla6	±	8.5 ± 2.0	0.2 ± 0.2
nla28	±	108.1 ± 2.1	2.1 ± 1.5
nla6 nla28	±	0.5 ± 0.2	0.02 ± 0.2

*Cells were placed on TPM [10 mM Tris-HCl, (pH 8.0), 1.0 mM KH₂PO₄, and 8 mM MgSO₄] agar and allowed to develop for 5 d. Mean values (±SDs) for the spore assays are shown as percentages of DK1622 (WT).

^†+, produced normal-looking fruiting bodies; ±, produced normal-looking fruiting bodies, but aggregation was delayed.

^‡These values were determined by counting the number of spherical-shaped spores using a Petroff–Hausser chamber and phase-contrast microscopy. Means (±SDs) derived from three independent experiments are shown.

^§These values were determined by transferring sonication- and heat-resistant spores to CTTYE agar plates, incubating the plates for 5 d, and counting the number of colonies that arose from the spores. Means (±SDs) derived from three independent experiments are shown.