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. Author manuscript; available in PMC: 2011 Aug 16.
Published in final edited form as: Biochemistry. 2006 Feb 28;45(8):2679–2685. doi: 10.1021/bi0522652

Figure 2.

Figure 2

SP-A-induced leakage of POPE containing liposomes is calcium dependent.

Incubation of fluorescent probe loaded POPE:LPS-J5 liposomes with 100 μg/ml of human SPA or 15 μg/ml melittin in the presence of calcium resulted in the release of fluorescent label into the extraliposomal space. In contrast, neither 100 μg/ml albumin, or the filtrate from the human SP-A reagent (3000 MWCO), had an effect on permeability of the POPE:LPS-J5 liposomes. Incorporation of cholesterol (25%) into the liposomal membrane blocked the susceptibility of POPE:LPS-J5 liposomes to permeabilization. Leakage of ANTS/DPX into the extraliposomal space was measured by fluorimetry as described under Materials and Methods. Data are expressed as means ± S.E., n=5. *, p<0.05.