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. Author manuscript; available in PMC: 2011 Aug 16.
Published in final edited form as: Biochemistry. 2006 Feb 28;45(8):2679–2685. doi: 10.1021/bi0522652

Figure 3.

Figure 3

Incorporation of cholesterol into rough LPS-J5 containing liposomes blocks SP-A-induced permeabilization.

Fluorescent microscopy of (A) POPE:LPS-J5 liposomes alone; (B) POPE:LPS-J5:cholesterol liposomes alone; (C) POPE:LPS-J5 liposomes upon exposure to 15 μg/ml melittin; (D) POPE:LPS-J5:cholesterol liposomes upon exposure to 15 μg/ml melittin; (E) POPE:LPS-J5 liposomes upon exposure to 100 μg/ml SP-A; (F) POPE:LPS-J5:cholesterol liposomes upon exposure to 100 μg/ml SP-A are shown. Experiments were performed in the presence of 1 mM CaCl2. 100× magnification was used.