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. Author manuscript; available in PMC: 2011 Aug 16.
Published in final edited form as: Biochemistry. 2006 Feb 28;45(8):2679–2685. doi: 10.1021/bi0522652

Figure 4.

Figure 4

Cholesterol blocks the SP-A-mediated permeabilization of POPE:LPS-J5 liposomes in a concentration-dependent manner.

Permeabilization of POPE:LPS-J5 liposomes containing various amounts of cholesterol in the presence of 100 μg/ml human SP-A (dotted line) or 15 μg/ml melittin (solid line) are shown in panel A. Loading of ANTS/DPX label into the cholesterol containing liposomes was more effective than for the cholesterol-free liposomes (panel B). Leakage of ANTS/DPX into the extraliposomal space was measured by fluorimetry as described under Materials and Methods. Data are means ± S.E., n=5. *, p<0.05. Experiments were performed in the presence of 1 mM CaCl2.