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Recombinant PCR as a means of generating a DNA fragment for use as a transcription template without the need for cloning. (A) Schematic of the PCR reactions needed to generate a full length fragment for transcription. Reactions 1a, 1b, and 1c are analogous to the reactions outlined in Sections 3.3.1, 3.3.2, and 3.3.3, respectively. (B) Ethidium bromide stained gel showing fragments generated in Step 1 (lane a, b) and Step 2 (lane c) of the scheme.
