Fig. 2.

Effect of 5-HMF on BBB permeability under acute hypobaric hypoxia using Evans blue in mice. The animals were exposed to normoxia or simulated sublethally acute hypobaric hypoxia of 8,300 m in a decompression chamber for 6 h after 1 h of 100 mg/kg 5-HMF or vehicle administration. The Evans blue extravasation assay was used to evaluate the BBB permeability and it was measured as described in the “Materials and methods” section. The hypoxia group demonstrated a significantly higher concentration of Evans blue in brain tissue than the control group. 5-HMF treatment decreased Evans blue extravasation in hypoxia-stressed group. The Evans blue extravasation is converted using a standardized curve to quantify the amount of Evans blue per brain weight (ng/mg). All data were expressed as means ± SD, n = 5; ^**p < 0.01, versus normoxia control; ^#p < 0.05, versus hypoxia control. Nor normoxia, Hyp hypoxia, 5-HMF 100 mg/kg 5-HMF