Fig. 2.

CD reverses cytotoxicity of cholesterol in MIN6. MIN6 cells were exposed to 250× diluted CLC (cho.) or 0.125% CD (CD) alone, or diluted CLC and CD together (cho./CD). The cell images were obtained with a phase-contrast microscope at 24 and 48 h after the exposure. Bar 25 μm (a). The numbers of adherent cells were expressed as percentage of the number of control cells (b). Mean ± SD (n = 3). *p < 0.05 vs. the levels of cholesterol loaded-cells (cho.). MIN6 cells were exposed to cholesterol with or without CD for 24 h, and then sterol contents in the whole cell extracts were determined by an enzymatic cholesterol assay kit (c). n = 3, mean ± SD; *p < 0.05 vs. the levels of contro; ^#p < 0.05 vs. the levels of cholesterol-loaded cells (cho.)