Table 3.
Gene | Size of a full-length transcript expected observed | Distance from the transcription start point to the downstream CodY-binding motif | Size of the shortest terminated transcript | |
---|---|---|---|---|
ybgE | 175 | 177 | 80b | 76 |
bcaP | 131 | 133 | 70 | 67 |
yufN | 308c | 309 | 168 | 164 |
gudBp2 | 235 | 233 | 124 | 120 |
DNA sequencing ladders were used to determine transcript sizes (in nucleotides). The deduced transcript size was calculated using the coefficient of 0.96, reflecting the average difference in mass between dNTPs and NTPs.
The distance to the proximal motif 2 of site II is specified.
The apparent yufN transcription start point was determined to be 270 bp upstream of the initiation codon (Belitsky and Sonenshein, manuscript in preparation).