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. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Vaccine. 2011 Jun 23;29(37):6292–6300. doi: 10.1016/j.vaccine.2011.06.027

Figure 4.

Figure 4

Evaluation of the specificity and isotype of serum antibody responses by ELISA. (A) The amount of anti-NR21 specific IgG1, IgG2a, and IgG2b subclass antibody was measured by quantitative ELISA in sera collected from BALB/c mice immunized with S. mutans alone or immunized with IC containing the Guy’s 13 plantibody. Antibody concentrations were extrapolated using standard curves. (B) The amount of anti-A3VP1 specific IgG1, IgG2a, and IgG2b subclass antibody was measured by quantitative ELISA in sera collected from BALB/c mice immunized with S. mutans alone or immunized with IC containing the Guy’s 13 plantibody. Antibody concentrations were extrapolated using standard curves. (C) Levels of total anti-S. mutans IgG in the sera from S. mutans versus Guy’s 13 plantibody IC-immunized mice were evaluated by serial endpoint titration and standard ELISA. The key for the line graphs indicates whether the mice received the PBS buffer control, S. mutans alone, or the dilution of the Guy’s 13 plantibody within the S. mutans IC used for immunization. All results are expressed as mean ± SEM and data are representative of at least three independent experiments. Statistically significant differences between groups receiving IC compared to S. mutans alone were determined my two-way ANOVA and are indicated by *(p≤0.05); **(p≤0.01); ***(p≤0001).