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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Dev Cell. 2011 Jul 28;21(2):301–314. doi: 10.1016/j.devcel.2011.06.033

Figure 1. Sema-PlxnD1 signaling is cell autonomously required within the endothelium for proper SeA sprout abundance and distribution.

Figure 1

(A–B) 23 hpf vasculatures, green. SBs, red. Horizontal myoseptum (HM), white dotted line. SeA sprouts, numbered. (A) WT. (B) obd. SeA sprout position (C) and abundance (D) in 23 hpf WT and obd. (E–F) WISH, 28 hpf trunks. Ectopic SeA sprouts, white arrowheads. Riboprobes: flt4 (blue), cdh5 (red). WT (E). obd (F). (G–H) 32 hpf chimeric vasculatures with ECs of donor (green) and host (red) origin. Examples of ectopic SeA sprouts, white arrowheads. (C–D) n = 8 WT, 12 obd. Error bars, s.e.m. ***p < 0.001. (E–F) n = 10/10 WT, 10/10 obd. (A–B, E–H) Anterior, left; dorsal, up. Scale bars, 30 μm. See Figure S1.