Skip to main content
. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Dev Cell. 2011 Jul 28;21(2):301–314. doi: 10.1016/j.devcel.2011.06.033

Figure 4. plxnD1 and sflt1 interact genetically, sflt1 limits SeA angiogenesis cell autonomously.

Figure 4

(A–I) 32 hpf trunk vasculatures, green. (A–E) SBs, red. White arrowheads, ectopic SeA sprouts. Blue arrowheads, ectopic SeA branching. (A, C, E) Embryos treated with 20 ng of sflt1-ctrl MO: WT (A), obd/+ (C), obd (E). Embryos treated with 20 ng of sflt1-splice MO: WT (B), obd/+ (D). (F) 23 hpf SeA sprout abundance in WT (left, grey bar) and obd/+ (right, black bar) sflt1-splice morphants. n = 20 WT and n = 19 obd/+. Error bars, s.e.m. ***p < 0.001. (G–I′) SBs, blue. GAL4FF/UAS-mediated endothelial-specific sflt1 over-expression, red. White arrows, missing SeA sprouts. (G′–H″) Endothelial sflt1 over-expression inhibits SeA sprouting. WT (G-G″). obd (H-H″), note lack of sflt1 over-expression (red) in remaining SeA sprout (white arrowhead). (I-I′) Mosaic vasculature with ECs from both obd donor and WT host. Endothelial-specific and mosaic sflt1 and DsRed co-expression restricted to the WT endothelium (red, I-I′). obd ECs express cytosolically-targeted EGFP (grey in I; green in I′). WT ECs express nuclear-targeted EGFP (white in I; green in I′). obd and WT ECs without sflt1 over-expression (DsRed-) from SeA sprouts even next to sflt1 over-expressing WT ECs (DsRed+). WT ECs over-expressing sflt1 (DsRed+) fail to form SeA sprouts (white arrows, I-I′). (G-H″) n = 30 embryos with over-expression per genotype, all showing suppression of SeA sprouting. Anterior, left; dorsal, up. Scale bars, 30 μm. See Figure S4.