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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Bioorg Med Chem Lett. 2011 Apr 20;21(17):5058–5061. doi: 10.1016/j.bmcl.2011.04.051

Figure 4.

Figure 4

Application of the sensor to monitor Cdc42 nucleotide exchange. Cdc42 was pre-equilibrated with GDP, and the fluorescence of sensor 3 was monitored immediately upon the addition of GTP-γS or GDP. Measurements were taken with 150 nM 4-DMN sensor 3 and 700 nM Cdc42 at 30 °C.