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Real-time qPCR for miRNAs. Graph indicates relative change in miRNA level. Total RNA was isolated from intact and crushed sciatic nerves (n=6). Relative quantitation of miRNA level was calculated against the reference gene (S12), and a relative fold change was determined using the 2 ΔΔCT method (n=3, *p<0.05). The levels of miR-221, miR-142-5p and miR-21 showed significant increase in sciatic nerve after crush.
