Table 1.

Regulation of miRNAs at 4 days after nerve crush

Total RNA for the microarray expression analysis was isolated from sciatic nerves, pooled from ten mice, at 4, and 7 days after the sciatic nerve crush. Both custom and Agilent arrays were done in duplicates and repeated two times including dye swap. Normalization and analyses were performed with GeneSpring software (Agilent). Data from custom and Agilent arrays were log2-transformed and median-centred by array. The GeneSpring analysis software was used for the identification of statistically significant upregulated or downregulated miRNAs at selected time points. The analysis also allowed for identification of a different temporal pattern of expression in the crushed groups compared with the control groups at the chosen time. For further analyses we selected three miRNAs that were significantly upregulated and one that was significantly downregulated in both custom arrays as well as in Agilent arrays.

Custom array	Fold Diff	Regulation	Agilent array	Fold Diff	Regulation	Average of both arrays	Fold Diff	Regulation
miR-424	2.05	up	miR-29b	2.14	up	miR-29b	2.26	up
miR-223	2.22	up	miR-142-5p	2.27	up	miR-142-5p	2.42	up
miR-29b	2.38	up	miR-221	2.63	up	miR-424	2.43	up
miR-142-5p	2.56	up	miR-223	2.70	up	miR-223	2.46	up
miR-221	2.77	up	miR-424	2.81	up	miR-221	2.70	up
miR-142-3p	5.21	up	miR-142-3p	4.56	up	miR-142-3p	4.88	up
miR-21	13.67	up	miR-21	13.04	up	miR-21	13.36	up
miR-133b	1.23	down	miR-99a	2.02	down	miR-99a	1.67	down
miR-99a	1.32	down	miR-124a	2.04	down	miR-30d	1.77	down
miR-30d	1.39	down	miR-125b	2.08	down	miR-143	1.86	down
miR-143	1.60	down	miR-100	2.09	down	miR-124a	1.92	down
miR-9*	1.66	down	miR-422b	2.11	down	miR-9*	1.94	down
miR-9	1.72	down	miR-143	2.12	down	miR-100	1.95	down
miR-124a	1.79	down	miR-30d	2.15	down	miR-422b	1.98	down
miR-100	1.81	down	miR-9*	2.22	down	miR-125b	2.03	down
miR-30a-3p	1.82	down	miR-138	2.40	down	miR-9	2.13	down
miR-422b	1.84	down	miR-338	2.44	down	miR-30a-3p	2.18	down
miR-150	1.95	down	miR-9	2.54	down	miR-338	2.24	down
miR-125b	1.98	down	miR-30a-3p	2.55	down	miR-138	2.26	down
miR-338	2.04	down	miR-145	2.75	down	miR-150	2.52	down
miR-182	2.07	down	miR-150	3.08	down	miR-145	2.64	down
miR-138	2.11	down	miR-183	3.23	down	miR-204	2.78	down
miR-204	2.32	down	miR-204	3.23	down	miR-182	2.79	down
miR-183	2.44	down	miR-182	3.51	down	miR-183	2.83	down
miR-145	2.53	down	miR-133b	4.47	down	miR-133b	2.85	down