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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cell Signal. 2011 Jun 25;23(11):1794–1805. doi: 10.1016/j.cellsig.2011.06.014

Figure 7. Effects of siRNA-mediated suppression of Rac1 and PAK family members on PRL-induced activation of ERK1/2.

Figure 7

MCF-7 cells were either left untrasfected (Control) or were transiently transfected in dublicates with non-targeting (NT) negative control siRNA, siRNA duplexes specific for Rac1, group I PAKs (PAK1/2/3), group II PAKs (PAK4/6/7) or group I/II PAKs for 72 hours. Cells were then stimulated with 10 nM PRL for 15 min. Phosphorylated forms of ERK1/2 (Thr202/Tyr204) were detected by IB of the TCL. GAPDH levels served as protein loading control. Representative blots are shown in the upper panel. The lower panel shows a graph with the calculated averages ±SD of remaining ERK1/2 phosphorylation (normalized to GAPDH) from two independent experiments.